CD19 Mouse Monoclonal Antibody [Clone ID: OTI2B11]

CAT#: CF506241

Carrier-free (BSA/glycerol-free) CD19 mouse monoclonal antibody, clone OTI2B11 (formerly 2B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19. Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

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  Anti-CD19 mouse monoclonal antibody (TA506241) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD19 (RC202922).

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  Living HEK293T cells transfected with either CD19 (RC202922) overexpression plasmid or empty vector control plasmid were immunostained by either anti-CD19 antibody (TA506241) or a nonspecific negative control antibody ([TA180143]) and then analyzed by flow cytometry. CD19 (red) or empty vector (blue) transfected cells with anti-CD19 antibody. CD19 (green) or empty vector (aqua) transfected cells with control antibody (1:100).

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  Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-huamn CD19 antibody (TA506241), (Red), compared to a nonspecific negative control antibody (TA180144), (green) and PBS, (blue) (1:20).

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  Flow cytometric Analysis of living Raji cells, using anti-CD19 antibody (TA506241), (Red), compared to a nonspecific negative control antibody [TA180143], (green), or PBS (blue) (1:20).

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Specifications

Product Data

• Clone Name: OTI2B11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD19(NP_001761) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.9 kDa
• Gene Name: CD19 molecule
• Database Link:
  NP_001761
  Entrez Gene 930 Human
  UniProt ID P15391
• Background: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
• Synonyms: B4; CVID3

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Hematopoietic cell lineage, Primary immunodeficiency