Hsc70 (HSPA8) Mouse Monoclonal Antibody [Clone ID: OTI1H3]

CAT#: CF505966

Carrier-free (BSA/glycerol-free) HSPA8 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSPA8 (Cat# RC202209, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA8(Cat# TA505966). Positive lysates LY401978 (100ug) and LC401978 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA8 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505966)

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  Anti-HSPA8 mouse monoclonal antibody (TA505966) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8 (RC202209).

Specifications

Product Data

• Clone Name: OTI1H3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSPA8(NP_006588) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.7 kDa
• Gene Name: Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.
• Database Link:
  NP_006588
  Entrez Gene 15481 MouseEntrez Gene 24468 RatEntrez Gene 3312 Human
  UniProt ID P11142
• Background: This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
• Synonyms: HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome