ICT1 (MRPL58) Mouse Monoclonal Antibody [Clone ID: OTI2F9]

CAT#: CF505881

Carrier-free (BSA/glycerol-free) ICT1 mouse monoclonal antibody, clone OTI2F9 (formerly 2F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ICT1 (Cat# RC204448, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICT1(Cat# TA505881). Positive lysates LY419875 (100ug) and LC419875 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ICT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505881)

Specifications

Product Data

• Clone Name: OTI2F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ICT1(NP_001536) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.4 kDa
• Gene Name: mitochondrial ribosomal protein L58
• Database Link:
  NP_001536
  Entrez Gene 3396 Human
  UniProt ID Q14197
• Background: The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is ICT1 whose mRNA and protein were more highly expressed in undifferentiated than in differentiated cells. [provided by RefSeq, Jul 2008]
• Synonyms: DS-1; DS1; ICT1; MRP-L58