SAPK4 (MAPK13) Mouse Monoclonal Antibody [Clone ID: OTI2G1]

CAT#: CF505852

Carrier-free (BSA/glycerol-free) MAPK13 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK13 (RC200606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK13. Positive lysates LY400973 (100ug) and LC400973 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK13 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-MAPK13 mouse monoclonal antibody (TA505852) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK13 (RC200606).

Specifications

Product Data

• Clone Name: OTI2G1
• Applications: IF, WB
• Recommended Dilution: WB 1:200~2000, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAPK13(NP_002745) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.9 kDa
• Gene Name: mitogen-activated protein kinase 13
• Database Link:
  NP_002745
  Entrez Gene 26415 MouseEntrez Gene 29513 RatEntrez Gene 612821 DogEntrez Gene 719085 MonkeyEntrez Gene 5603 Human
  UniProt ID O15264
• Synonyms: MAPK-13; MAPK 13; p38delta; PRKM13; SAPK4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway