RPC62 (POLR3C) Mouse Monoclonal Antibody [Clone ID: OTI2H1]

CAT#: CF505106

Carrier-free (BSA/glycerol-free) POLR3C mouse monoclonal antibody, clone OTI2H1 (formerly 2H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POLR3C (Cat# RC200279, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR3C(Cat# TA505106). Positive lysates LY401943 (100ug) and LC401943 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-POLR3C monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-POLR3C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505106)

• Anti-POLR3C mouse monoclonal antibody (TA505106) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR3C (RC200279).

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Specifications

Product Data

• Clone Name: OTI2H1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POLR3C(NP_006459) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.4 kDa
• Gene Name: RNA polymerase III subunit C
• Database Link:
  NP_006459
  Entrez Gene 74414 MouseEntrez Gene 310685 RatEntrez Gene 10623 Human
  UniProt ID Q9BUI4
• Synonyms: RPC3; RPC62

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase