DREF (ZBED1) Mouse Monoclonal Antibody [Clone ID: OTI5A2]

CAT#: CF505044

Carrier-free (BSA/glycerol-free) ZBED1 mouse monoclonal antibody, clone OTI5A2 (formerly 5A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZBED1 (Cat# RC202011, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZBED1(Cat# TA505044). Positive lysates LY417799 (100ug) and LC417799 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505044)

• HEK293T cells transfected with either RC202011 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ZBED1 antibody (TA505044), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5A2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZBED1(NP_004720) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78 kDa
• Gene Name: zinc finger BED-type containing 1
• Database Link:
  NP_004720
  Entrez Gene 9189 Human
  UniProt ID O96006
• Background: This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. [provided by RefSeq]
• Synonyms: ALTE; DREF; hDREF; TRAMP

Reference Data

• Protein Families: Druggable Genome