Tumor protein D52 like 3 (TPD52L3) Mouse Monoclonal Antibody [Clone ID: OTI3D11]

CAT#: CF504985

Carrier-free (BSA/glycerol-free) TPD52L3 mouse monoclonal antibody, clone OTI3D11 (formerly 3D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPD52L3 (RC205471, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPD52L3. Positive lysates LY424258 (100ug) and LC424258 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TPD52L3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504985)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TPD52L3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504985)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPD52L3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504985)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TPD52L3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504985)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TPD52L3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504985)

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Specifications

Product Data

• Clone Name: OTI3D11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protien of human TPD52L3(NP_001001875) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.4 kDa
• Gene Name: TPD52 like 3
• Database Link:
  NP_001001875
  Entrez Gene 89882 Human
  UniProt ID Q96J77
• Background: This gene encodes a member of the tumor protein D52-like family of proteins. These proteins are characterized by an N-terminal coiled-coil motif that is used to form homo- and heteromeric complexes with other tumor protein D52-like proteins. The encoded protein may play a role in spermatogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
• Synonyms: hD55; NYDSP25

Reference Data

• Protein Families: Druggable Genome