CRYZL1 Mouse Monoclonal Antibody [Clone ID: OTI2F9]

CAT#: CF504659

Carrier-free (BSA/glycerol-free) CRYZL1 mouse monoclonal antibody, clone OTI2F9 (formerly 2F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CRYZL1 (Cat# RC205953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYZL1(Cat# TA504659). Positive lysates LY407853 (100ug) and LC407853 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRYZL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  HEK293T cells transfected with either RC205953 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYZL1 antibody (TA504659), and then analyzed by flow cytometry.

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  Anti-CRYZL1 mouse monoclonal antibody (TA504659) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYZL1 (RC205953).

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CRYZL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504659)

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Specifications

Product Data

• Clone Name: OTI2F9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CRYZL1(NP_665857) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.5 kDa
• Gene Name: crystallin zeta like 1
• Database Link:
  NP_665857
  Entrez Gene 66609 MouseEntrez Gene 288256 RatEntrez Gene 698723 MonkeyEntrez Gene 9946 Human
  UniProt ID O95825
• Background: This gene encodes a protein that has sequence similarity to zeta crystallin, also known as quinone oxidoreductase. This zeta crystallin-like protein also contains an NAD(P)H binding site. Alternatively spliced transcript variants have been observed but their full-length nature has not been completely determined. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: 4P11; QOH-1

Reference Data

• Protein Families: Druggable Genome