PALS1 Mouse Monoclonal Antibody [Clone ID: OTI5E10]

CAT#: CF504459

Carrier-free (BSA/glycerol-free) MPP5 mouse monoclonal antibody, clone OTI5E10 (formerly 5E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPP5 (RC224752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPP5. Positive lysates LY411659 (100ug) and LC411659 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5E10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MPP5(NP_071919) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.1 kDa
• Gene Name: Homo sapiens membrane palmitoylated protein 5 (MPP5), transcript variant 1, mRNA.
• Database Link:
  NP_071919
  Entrez Gene 314259 RatEntrez Gene 64398 Human
  UniProt ID Q8N3R9
• Background: Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001 [PubMed 11311936]). MPP5 is a member of the p55-like MAGUK subfamily. [supplied by OMIM]
• Synonyms: PALS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Tight junction