PTOP (ACD) Mouse Monoclonal Antibody [Clone ID: OTI1C8]

CAT#: CF504201

Carrier-free (BSA/glycerol-free) ACD mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACD (RC204381, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACD. Positive lysates LY421183 (100ug) and LC421183 (20ug) can be purchased separately from OriGene.

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  Anti-ACD mouse monoclonal antibody (TA504201) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACD (RC204381).

Specifications

Product Data

• Clone Name: OTI1C8
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACD(NP_001075955) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.6 kDa
• Gene Name: ACD shelterin complex subunit and telomerase recruitment factor
• Database Link:
  NP_001075955
  Entrez Gene 65057 Human
  UniProt ID Q96AP0
• Background: This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I. [provided by RefSeq]
• Synonyms: PIP1; PTOP; TINT1; TPP1