MKRN1 Mouse Monoclonal Antibody [Clone ID: OTI2C8]

CAT#: CF504092

Carrier-free (BSA/glycerol-free) MKRN1 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MKRN1 (Cat# RC206848, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1(Cat# TA504092). Positive lysates LY415580 (100ug) and LC415580 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MKRN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA504092)

• Flow cytometric Analysis of Hela cells, using anti-MKRN1 antibody (TA504092), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-MKRN1 antibody (TA504092), (Red), compared to a nonspecific negative control antibody, (Blue).

Show all 14 images

Specifications

Product Data

• Clone Name: OTI2C8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recomnbinant protein of human MKRN1(NP_038474) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.2 kDa
• Gene Name: Homo sapiens makorin ring finger protein 1 (MKRN1), transcript variant 1, mRNA.
• Database Link:
  NP_038474
  Entrez Gene 54484 MouseEntrez Gene 23608 Human
  UniProt ID Q9UHC7
• Background: The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family (Gray et al., 2000 [PubMed 10843807]). [supplied by OMIM, Mar 2008]
• Synonyms: RNF61

Reference Data

• Protein Families: Druggable Genome