HARS2 Mouse Monoclonal Antibody [Clone ID: OTI6F9]

CAT#: CF503866

Carrier-free (BSA/glycerol-free) HARS2 mouse monoclonal antibody, clone OTI6F9 (formerly 6F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HARS2 (Cat# RC204925, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HARS2(Cat# TA503866). Positive lysates LY415917 (100ug) and LC415917 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HARS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503866)

• HEK293T cells transfected with either RC204925 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HARS2 antibody (TA503866), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6F9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HARS2(NP_036340) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.3 kDa
• Gene Name: histidyl-tRNA synthetase 2, mitochondrial
• Database Link:
  NP_036340
  Entrez Gene 23438 Human
  UniProt ID P49590
• Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes share a bidirectional promoter. [provided by RefSeq]. COMPLETENESS: full length.
• Synonyms: HARSL; HARSR; HO3; PRLTS2

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis