ARH (LDLRAP1) Mouse Monoclonal Antibody [Clone ID: OTI5A12]

CAT#: CF503851

Carrier-free (BSA/glycerol-free) LDLRAP1 mouse monoclonal antibody, clone OTI5A12 (formerly 5A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDLRAP1 (RC206643, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDLRAP1. Positive lysates LY414462 (100ug) and LC414462 (20ug) can be purchased separately from OriGene.

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  Anti-LDLRAP1 mouse monoclonal antibody (TA503851) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LDLRAP1 (RC206643).

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  HEK293T cells transfected with either RC206643 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LDLRAP1 antibody (TA503851), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5A12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LDLRAP1(NP_056442) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: low density lipoprotein receptor adaptor protein 1
• Database Link:
  NP_056442
  Entrez Gene 100017 MouseEntrez Gene 500564 RatEntrez Gene 26119 Human
  UniProt ID Q5SW96
• Background: The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq, Jul 2008]
• Synonyms: ARH; ARH1; ARH2; FHCB1; FHCB2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis