Adiponectin (ADIPOQ) Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: CF503801

Carrier-free (BSA/glycerol-free) ADIPOQ mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ADIPOQ (Cat# RC215161, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADIPOQ(Cat# TA503801). Positive lysates LY401510 (100ug) and LC401510 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-ADIPOQ monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503801)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503801)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503801)

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  Anti-ADIPOQ mouse monoclonal antibody (TA503801) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADIPOQ (RC215161).

• HEK293T cells transfected with either RC215161 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADIPOQ antibody (TA503801), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1B2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-244 of human ADIPOQ(NP_004788) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.9 kDa
• Gene Name: adiponectin, C1Q and collagen domain containing
• Database Link:
  NP_004788
  Entrez Gene 9370 Human
  UniProt ID Q15848
• Background: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
• Synonyms: ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus