Lipoprotein lipase (LPL) Mouse Monoclonal Antibody [Clone ID: OTI4F6]

CAT#: CF503792

Carrier-free (BSA/glycerol-free) LPL mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LPL (RC203766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPL. Positive lysates LY400089 (100ug) and LC400089 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LPL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503792)

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  Anti-LPL mouse monoclonal antibody (TA503792) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LPL (RC203766).

Specifications

Product Data

• Clone Name: OTI4F6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-475 of human LPL(NP_000228) produced in HEK293T cell
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.3 kDa
• Gene Name: lipoprotein lipase
• Database Link:
  NP_000228
  Entrez Gene 16956 MouseEntrez Gene 24539 RatEntrez Gene 4023 Human
  UniProt ID P06858
• Background: LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq]
• Synonyms: HDLCQ11; LIPD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Glycerolipid metabolism, PPAR signaling pathway