PNPO Mouse Monoclonal Antibody [Clone ID: OTI1H9]

CAT#: CF503356

Carrier-free (BSA/glycerol-free) PNPO mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO. Positive lysates LY413282 (100ug) and LC413282 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PNPO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503356)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503356)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503356)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503356)

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Specifications

Product Data

• Clone Name: OTI1H9
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa
• Gene Name: Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), mRNA.
• Database Link:
  NP_060599
  Entrez Gene 64533 RatEntrez Gene 103711 MouseEntrez Gene 55163 Human
  UniProt ID Q9NVS9
• Background: The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: HEL-S-302; PDXPO

Reference Data

• Protein Pathways: Metabolic pathways, Vitamin B6 metabolism