Nudel (NDEL1) Mouse Monoclonal Antibody [Clone ID: OTI5E11]

CAT#: CF503353

Carrier-free (BSA/glycerol-free) NDEL1 mouse monoclonal antibody, clone OTI5E11 (formerly 5E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NDEL1 (Cat# RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1(Cat# TA503353). Positive lysates LY422448 (100ug) and LC422448 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDEL1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503353)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503353)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503353)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503353)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503353)

• Anti-NDEL1 mouse monoclonal antibody (TA503353) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1 (RC212323).

• HEK293T cells transfected with either RC212323 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDEL1 antibody (TA503353), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5E11
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NDEL1(NP_001020750) produced in HEk293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.9 kDa
• Gene Name: nudE neurodevelopment protein 1 like 1
• Database Link:
  NP_001020750
  Entrez Gene 83431 MouseEntrez Gene 170845 RatEntrez Gene 81565 Human
  UniProt ID Q9GZM8
• Background: This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
• Synonyms: EOPA; MITAP1; NDE1L1; NDE2; NUDEL