PDE1B Mouse Monoclonal Antibody [Clone ID: OTI1D6]

CAT#: CF503336

Carrier-free (BSA/glycerol-free) PDE1B mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (RC206588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B. Positive lysates LY400337 (100ug) and LC400337 (20ug) can be purchased separately from OriGene.

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  Anti-PDE1B mouse monoclonal antibody (TA503336) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B (RC206588).

Specifications

Product Data

• Clone Name: OTI1D6
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.2 kDa
• Gene Name: phosphodiesterase 1B
• Database Link:
  NP_000915
  Entrez Gene 18574 MouseEntrez Gene 29691 RatEntrez Gene 5153 Human
  UniProt ID Q01064
• Background: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
• Synonyms: PDE1B1; PDES1B

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism