PDE1B Mouse Monoclonal Antibody [Clone ID: OTI6B9]

CAT#: CF503325

Carrier-free (BSA/glycerol-free) PDE1B mouse monoclonal antibody, clone OTI6B9 (formerly 6B9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (RC206588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B. Positive lysates LY400337 (100ug) and LC400337 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody.

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  Anti-PDE1B mouse monoclonal antibody (TA503325) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B (RC206588).

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  HEK293T cells transfected with either RC206588 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE1B antibody (TA503325), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI6B9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.2 kDa
• Gene Name: phosphodiesterase 1B
• Database Link:
  NP_000915
  Entrez Gene 18574 MouseEntrez Gene 29691 RatEntrez Gene 100856025 DogEntrez Gene 705920 MonkeyEntrez Gene 5153 Human
  UniProt ID Q01064
• Background: Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]). [supplied by OMIM]
• Synonyms: PDE1B1; PDES1B

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism