SETD7 Mouse Monoclonal Antibody [Clone ID: OTI2D10]

CAT#: CF503322

Carrier-free (BSA/glycerol-free) SETD7 mouse monoclonal antibody, clone OTI2D10 (formerly 2D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SETD7 (Cat# RC219244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7(Cat# TA503322). Positive lysates LY403067 (100ug) and LC403067 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody.

• 

  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SETD7 monoclonal antibody (1:200).

• 

  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-SETD7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SETD7-Knockout HeLa cells (KO, Cat# LC833317) were separated by SDS-PAGE and immunoblotted with anti-SETD7 monoclonal antibody TA503322 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503322)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503322)

• Anti-SETD7 mouse monoclonal antibody (TA503322) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SETD7 (RC219244).

• HEK293T cells transfected with either RC219244 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SETD7 antibody (TA503322), and then analyzed by flow cytometry.

Show all 9 images

Specifications

Product Data

• Clone Name: OTI2D10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SETD7(NP_085151) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.5 kDa
• Gene Name: Homo sapiens SET domain containing 7, histone lysine methyltransferase (SETD7), transcript variant 1, mRNA.
• Database Link:
  NP_085151
  Entrez Gene 73251 MouseEntrez Gene 689954 RatEntrez Gene 476079 DogEntrez Gene 700426 MonkeyEntrez Gene 80854 Human
  UniProt ID Q8WTS6
• Synonyms: KMT7; SET7; SET7/9; SET9

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation