Aminoacylase 1 (ACY1) Mouse Monoclonal Antibody [Clone ID: OTI1B12]

CAT#: CF503227

Carrier-free (BSA/glycerol-free) ACY1 mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (RC201284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1. Positive lysates LY424578 (100ug) and LC424578 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACY1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503227)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503227)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ACY1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503227)

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  HEK293T cells transfected with either RC201284 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody (TA503227), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1B12
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACY1(NP_000657) produced in HEk293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.7 kDa
• Gene Name: aminoacylase 1
• Database Link:
  NP_000657
  Entrez Gene 109652 MouseEntrez Gene 300981 RatEntrez Gene 476602 DogEntrez Gene 95 Human
  UniProt ID Q03154
• Background: This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq]
• Synonyms: ACY-1; ACY1D; HEL-S-5

Reference Data

• Protein Families: Protease
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways