SOCS3 Mouse Monoclonal Antibody [Clone ID: OTI6F3]

CAT#: CF502922

Carrier-free (BSA/glycerol-free) SOCS3 mouse monoclonal antibody, clone OTI6F3 (formerly 6F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (RC209305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.

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  HEK293T cells transfected with either RC209305 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SOCS3 antibody (TA502922), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-SOCS3 antibody (TA502922), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI6F3
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SOCS3 (NP_003946) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.6 kDa
• Gene Name: Homo sapiens suppressor of cytokine signaling 3 (SOCS3), transcript variant 1, mRNA.
• Database Link:
  NP_003946
  Entrez Gene 12702 MouseEntrez Gene 89829 RatEntrez Gene 9021 Human
  UniProt ID O14543
• Background: This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq]
• Synonyms: ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Type II diabetes mellitus, Ubiquitin mediated proteolysis