LMCD1 Mouse Monoclonal Antibody [Clone ID: OTI2G5]

CAT#: CF502790

Carrier-free (BSA/glycerol-free) LMCD1 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (RC200062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1. Positive lysates LY402349 (100ug) and LC402349 (20ug) can be purchased separately from OriGene.

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  Anti-LMCD1 mouse monoclonal antibody (TA502790) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMCD1 (RC200062 ).

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  HEK293T cells transfected with either RC200062 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody (TA502790), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2G5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LMCD1 (NP_055398) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.7 kDa
• Gene Name: LIM and cysteine rich domains 1
• Database Link:
  NP_055398
  Entrez Gene 29995 Human
  UniProt ID Q9NZU5
• Background: The protein encoded by this gene contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. The presence of LIM domains implies involvement in protein-protein interactions. Expression of this gene has been detected in most tissues, with highest expression in skeletal muscle. Transcript variants utilizing alternative polyA signals have been observed. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: dyxin; LIM and cysteine-rich domains 1