POLR2E Mouse Monoclonal Antibody [Clone ID: OTI3B5]

CAT#: CF502548

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Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)



  View other "OTI3B5" antibodies (4)

USD 600.00


Availability*
In Stock

Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI3B5
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:500~2000, IHC 1:150, FLOW 1:100, IF: 1:100
Reactivities Human, Monkey, Mouse, Rat, Dog
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 24.4 kDa
Gene Name Homo sapiens RNA polymerase II, I and III subunit E (POLR2E), transcript variant 1, mRNA.
Background This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]
Synonyms hRPB25; hsRPB5; RPABC1; RPB5; XAP4
Reference Data
Protein Families Transcription Factors
Protein Pathways Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

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