GBA3 Mouse Monoclonal Antibody [Clone ID: OTI5G5]

CAT#: CF502533

Carrier-free (BSA/glycerol-free) GBA3 mouse monoclonal antibody, clone OTI5G5 (formerly 5G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (RC211035, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3. Positive lysates LY402815 (100ug) and LC402815 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-GBA3 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502533)

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  HEK293T cells transfected with either RC211035 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBA3 antibody (TA502533), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-GBA3 antibody (TA502533), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI5G5
• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3(NP_066024) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: glucosylceramidase beta 3 (gene/pseudogene)
• Database Link:
  NP_066024
  Entrez Gene 57733 Human
  UniProt ID Q9H227
• Background: GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]). [supplied by OMIM]
• Synonyms: CBG; CBGL1; GLUC; KLRP

Reference Data

• Protein Pathways: Cyanoamino acid metabolism, Starch and sucrose metabolism