POLR2E Mouse Monoclonal Antibody [Clone ID: OTI2A11]

CAT#: CF502490

Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody, clone OTI2A11 (formerly 2A11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E. Positive lysates LY419161 (100ug) and LC419161 (20ug) can be purchased separately from OriGene.

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  Anti-POLR2E mouse monoclonal antibody (TA502490) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E (RC201266).

Specifications

Product Data

• Clone Name: OTI2A11
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POLR2E (NP_002686) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.4 kDa
• Gene Name: RNA polymerase II, I and III subunit E
• Database Link:
  NP_002686
  Entrez Gene 66420 MouseEntrez Gene 690966 RatEntrez Gene 5434 Human
  UniProt ID P19388
• Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]
• Synonyms: hRPB25; hsRPB5; RPABC1; RPB5; XAP4

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase