PKC epsilon (PRKCE) Mouse Monoclonal Antibody [Clone ID: OTI1B7]

CAT#: CF502395

Carrier-free (BSA/glycerol-free) PRKCE mouse monoclonal antibody, clone OTI1B7 (formerly 1B7)

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKCE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:1000).

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502395)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502395)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKCE mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502395)

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  Anti-PRKCE mouse monoclonal antibody (TA502395) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKCE (RC217702).

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  HEK293T cells transfected with either RC217702 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKCE antibody (TA502395), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-PRKCE antibody (TA502395), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83.5 kDa
• Gene Name: Homo sapiens protein kinase C epsilon (PRKCE), mRNA.
• Database Link:
  NP_005391
  Entrez Gene 18754 MouseEntrez Gene 29340 RatEntrez Gene 609934 DogEntrez Gene 714533 MonkeyEntrez Gene 5581 Human
  UniProt ID Q02156
• Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]
• Synonyms: nPKC-epsilon; PKCE

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction