Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI6G5]

CAT#: CF501825

Carrier-free (BSA/glycerol-free) GAS7 mouse monoclonal antibody, clone OTI6G5 (formerly 6G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7. Positive lysates LY418527 (100ug) and LC418527 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GAS7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501825)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAS7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501825)

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  Anti-GAS7 mouse monoclonal antibody (TA501825) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GAS7 (RC215256).

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  Flow cytometric Analysis of Hela cells, using anti-GAS7 antibody (TA501825), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-GAS7 antibody (TA501825), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI6G5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GAS7 (NP_003635) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.0 kDa
• Gene Name: growth arrest specific 7
• Database Link:
  NP_003635
  Entrez Gene 14457 MouseEntrez Gene 85246 RatEntrez Gene 8522 Human
  UniProt ID O60861
• Background: Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described. [provided by RefSeq]
• Synonyms: GAS7; MLL

Reference Data

• Protein Families: Transcription Factors