alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: OTI2E2]

CAT#: CF501788

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Carrier-free (BSA/glycerol-free) AFP mouse monoclonal antibody, clone OTI2E2 (formerly 2E2)



  View other "OTI2E2" antibodies (5)

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USD 495.00


Availability*
In Stock

Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI2E2
Applications FC, IF, WB
Recommended Dilution WB: 1:200 - 1:1000, IF 1:100, FLOW 1:100
Reactivity Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 66.3 kDa
Gene Name Homo sapiens alpha fetoprotein (AFP), transcript variant 1, mRNA.
Background This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq]
Synonyms AFPD; FETA; HPAFP
Reference Data
Protein Families Druggable Genome, Secreted Protein

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