ARHGAP25 Mouse Monoclonal Antibody [Clone ID: OTI12G4]

CAT#: CF501745

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Carrier-free (BSA/glycerol-free) ARHGAP25 mouse monoclonal antibody, clone OTI12G4 (formerly 12G4)



  View other "OTI12G4" antibodies (4)

USD 545.00


Availability*
4 Weeks

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI12G4
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 72.3 kDa
Gene Name Homo sapiens Rho GTPase activating protein 25 (ARHGAP25), transcript variant 2, mRNA.
Background ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). [supplied by OMIM]
Synonyms HEL-S-308; KAIA0053
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.