NSE2 (NSMCE2) Mouse Monoclonal Antibody [Clone ID: OTI6A6]

CAT#: CF501635

Carrier-free (BSA/glycerol-free) NSMCE2 mouse monoclonal antibody, clone OTI6A6 (formerly 6A6)

Product Images

Anti-NSMCE2 mouse monoclonal antibody (TA501635) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NSMCE2 (RC207639).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSMCE2 (RC207639, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSMCE2. Positive lysates LY406558 (100ug) and LC406558 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC207639 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NSMCE2 antibody (TA501635), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-NSMCE2 antibody (TA501635), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI6A6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NSMCE2 (NP_775956) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.8 kDa
• Gene Name: NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase
• Database Link:
  NP_775956
  Entrez Gene 286053 Human
  UniProt ID Q96MF7
• Synonyms: C8orf36; MMS21; NSE2; ZMIZ7