NT5DC1 Mouse Monoclonal Antibody [Clone ID: OTI2A1]

CAT#: CF501591

Carrier-free (BSA/glycerol-free) NT5DC1 mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NT5DC1 (RC211087, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NT5DC1. Positive lysates LY403486 (100ug) and LC403486 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NT5DC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501591)

• HEK293T cells transfected with either RC211087 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NT5DC1 antibody (TA501591), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2A1
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NT5DC1 (NP_689942) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.7 kDa
• Gene Name: 5'-nucleotidase domain containing 1
• Database Link:
  NP_689942
  Entrez Gene 221294 Human
  UniProt ID Q5TFE4
• Background: While the exact function of the protein encoded by this gene is not known, it belongs to the 5'(3')-deoxyribonucleotidase family. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: C6orf200; LP2642; NT5C2L1