BTN1A1 Mouse Monoclonal Antibody [Clone ID: OTI5E6]

CAT#: CF501536

Carrier-free (BSA/glycerol-free) BTN1A1 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (RC223852, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1. Positive lysates LY400653 (100ug) and LC400653 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BTN1A1 monoclonal antibody.

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  Anti-BTN1A1 mouse monoclonal antibody (TA501536) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1 (RC223852).

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  HEK293T cells transfected with either RC223852 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody (TA501536), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5E6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTN1A1 (NP_001723) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.8 kDa
• Gene Name: butyrophilin subfamily 1 member A1
• Database Link:
  NP_001723
  Entrez Gene 306956 RatEntrez Gene 100855656 DogEntrez Gene 700608 MonkeyEntrez Gene 696 Human
  UniProt ID Q13410
• Background: Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families [provided by RefSeq].
• Synonyms: BT; BTN; BTN1

Reference Data

• Protein Families: Druggable Genome, Transmembrane