MOBKL2B (MOB3B) Mouse Monoclonal Antibody [Clone ID: OTI3A8]

CAT#: CF501505

Carrier-free (BSA/glycerol-free) MOBKL2B mouse monoclonal antibody, clone OTI3A8 (formerly 3A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MOBKL2B (RC205977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MOBKL2B. Positive lysates LY403025 (100ug) and LC403025 (20ug) can be purchased separately from OriGene.

• 

  Anti-MOBKL2B mouse monoclonal antibody (TA501505) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MOBKL2B (RC205977).

• 

  Flow cytometric analysis of HEK293T cells transfected with either RC205977 plasmid (Red) or empty vector control plasmid (Blue) using anti-MOBKL2B antibody (TA501505) at 1:100 dilution.

Specifications

Product Data

• Clone Name: OTI3A8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MOBKL2B (NP_079037) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.3 kDa
• Gene Name: MOB kinase activator 3B
• Database Link:
  NP_079037
  Entrez Gene 214944 MouseEntrez Gene 79817 Human
  UniProt ID Q86TA1
• Background: The protein encoded by this gene shares similarity with the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. This gene is located on the opposite strand as the interferon kappa precursor (IFNK) gene. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: C9orf35; MOB1D; MOBKL2B