MIOX Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CAT#: CF501454

Carrier-free (BSA/glycerol-free) MIOX mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIOX (RC210070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIOX. Positive lysates LY413679 (100ug) and LC413679 (20ug) can be purchased separately from OriGene.

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  Anti-MIOX mouse monoclonal antibody (TA501454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MIOX (RC210070).

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  HEK293T cells transfected with either RC210070 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIOX antibody (TA501454), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-MIOX antibody (TA501454), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI1C5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MIOX(NP_060054) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.8 kDa
• Gene Name: myo-inositol oxygenase
• Database Link:
  NP_060054
  Entrez Gene 55586 Human
  UniProt ID Q9UGB7
• Synonyms: ALDRL6

Reference Data

• Protein Pathways: Ascorbate and aldarate metabolism, Inositol phosphate metabolism