Coronin 1a (CORO1A) Mouse Monoclonal Antibody [Clone ID: OTI1A5]

CAT#: CF501429

Carrier-free (BSA/glycerol-free) CORO1A mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CORO1A (Cat# RC210753, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CORO1A (Cat# TA501429). Positive lysates LY402085 (100ug) and LC402085 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CORO1A monoclonal antibody.

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  Anti-CORO1A mouse monoclonal antibody (TA501429) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CORO1A (RC210753 ).

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  HEK293T cells transfected with either RC210753 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CORO1A antibody (TA501429), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-CORO1A antibody (TA501429), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-CORO1A antibody (TA501429), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1A5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CORO1A(NP_009005) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.8 kDa
• Gene Name: coronin 1A
• Database Link:
  NP_009005
  Entrez Gene 12721 MouseEntrez Gene 155151 RatEntrez Gene 11151 Human
  UniProt ID P31146
• Background: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq]
• Synonyms: CLABP; CLIPINA; HCORO1; IMD8; p57; TACO