BDH2 Mouse Monoclonal Antibody [Clone ID: OTI2G1]

CAT#: CF501293

Carrier-free (BSA/glycerol-free) BDH2 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BDH2 (RC210586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BDH2. Positive lysates LY402755 (100ug) and LC402755 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501293, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501293, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501293, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-BDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501293, Dilution 1:50)

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  Anti-BDH2 mouse monoclonal antibody (TA501293) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BDH2 (RC210586).

• HEK293T cells transfected with either RC210586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BDH2 antibody (TA501293), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2G1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BDH2(NP_064524) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.5 kDa
• Gene Name: 3-hydroxybutyrate dehydrogenase 2
• Database Link:
  NP_064524
  Entrez Gene 69772 MouseEntrez Gene 295458 RatEntrez Gene 56898 Human
  UniProt ID Q9BUT1
• Synonyms: DHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies