Hsp22 (HSPB8) Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CAT#: CF501210

Carrier-free (BSA/glycerol-free) HSPB8 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSPB8 (Cat# RC201040, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPB8 (Cat# TA501210). Positive lysates LY415329 (100ug) and LC415329 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPB8 monoclonal antibody.

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  Anti-HSPB8 mouse monoclonal antibody (TA501210) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPB8 (RC201040 ).

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  HEK293T cells transfected with either RC201040 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HSPB8 antibody (TA501210), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-HSPB8 antibody (TA501210), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1E3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSPB8(NP_055180) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.4 kDa
• Gene Name: heat shock protein family B (small) member 8
• Database Link:
  NP_055180
  Entrez Gene 80888 MouseEntrez Gene 113906 RatEntrez Gene 26353 Human
  UniProt ID Q9UJY1
• Background: The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq]
• Synonyms: CMT2L; DHMN2; E2IG1; H11; HMN2; HMN2A; HSP22

Reference Data

• Protein Families: Druggable Genome, Protein Kinase