hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5]

CAT#: CF500804

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Carrier-free (BSA/glycerol-free) HNRNPF mouse monoclonal antibody, clone OTI5F5 (formerly 5F5)

Formulation: Standard Carrier-Free



USD 600.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI5F5
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 45.7 kDa
Gene Name Homo sapiens heterogeneous nuclear ribonucleoprotein F (HNRNPF), transcript variant 3, mRNA.
Background This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Synonyms HNRPF; mcs94-1; OK/SW-cl.23
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.