IBRDC2 (RNF144B) Mouse Monoclonal Antibody [Clone ID: OTI9H10]

CAT#: CF500704

Carrier-free (BSA/glycerol-free) RNF144B mouse monoclonal antibody, clone OTI9H10 (formerly 9H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RNF144B (Cat# RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B(Cat# TA500704). Positive lysates LY405351 (100ug) and LC405351 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500704)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500704)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500704)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500704)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RNF144B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500704)

• Anti-RNF144B mouse monoclonal antibody (TA500704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B (RC209302).

• Immunofluorescent staining of HeLa cells using anti-RNF144B mouse monoclonal antibody (TA500704).

• HEK293T cells transfected with either RC209302 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RNF144B antibody (TA500704), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody (TA500704), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody (TA500704), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI9H10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:50~100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.5 kDa
• Gene Name: ring finger protein 144B
• Database Link:
  NP_877434
  Entrez Gene 218215 MouseEntrez Gene 255488 Human
  UniProt ID Q7Z419
• Synonyms: bA528A10.3; IBRDC2; p53RFP; PIR2

Reference Data

• Protein Families: Transmembrane