Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI10D7]

CAT#: CF500630

Carrier-free (BSA/glycerol-free) HSPA9 mouse monoclonal antibody, clone OTI10D7 (formerly 10D7)

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-HSPA9 mouse monoclonal antibody (TA500630) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 (RC201397).

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  HEK293T cells transfected with either RC201397 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HSPA9 antibody (TA500630), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI10D7
• Applications: FC, IF, WB
• Recommended Dilution: IF 1:100, FLOW 1:100, WB: 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.5 kDa
• Gene Name: heat shock protein family A (Hsp70) member 9
• Database Link:
  NP_004125
  Entrez Gene 15526 MouseEntrez Gene 291671 RatEntrez Gene 3313 Human
  UniProt ID P38646
• Background: This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
• Synonyms: CSA; GRP-75; GRP75; HEL-S-124m; HSPA9B; MOT; MOT2; MTHSP75; PBP74

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation