Aurora C (AURKC) Mouse Monoclonal Antibody [Clone ID: OTI2B7]

CAT#: CF500602

Carrier-free (BSA/glycerol-free) AURKC mouse monoclonal antibody, clone OTI2B7 (formerly 2B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AURKC (RC213890, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AURKC. Positive lysates LY423127 (100ug) and LC423127 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-AURKC monoclonal antibody (1:200).

Specifications

Product Data

• Clone Name: OTI2B7
• Applications: WB
• Recommended Dilution: WB: 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human AURKC expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.6 kDa
• Gene Name: aurora kinase C
• Database Link:
  NP_001015878
  Entrez Gene 6795 Human
  UniProt ID Q9UQB9
• Background: This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.
• Synonyms: AIE2; AIK3; ARK3; AurC; aurora-C; HEL-S-90; SPGF5; STK13

Reference Data

• Protein Families: Druggable Genome, Protein Kinase