DHFR Mouse Monoclonal Antibody [Clone ID: OTI6G7]

CAT#: CF500543

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Carrier-free (BSA/glycerol-free) DHFR mouse monoclonal antibody, clone OTI6G7 (formerly 6G7)


Product Datasheet for 'CF500543'


  View other "OTI6G7" antibodies (2)

USD 465.00


Availability*
4 Weeks

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI6G7
Applications IF, IHC, WB
Recommended Dilution WB 1:2000 IHC 1:50 IF 1:100
Reactivity Human, Monkey, Dog
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full-length protein expressed in 293T cell transfected with human DHFR expression vector
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 21.5 kDa
Gene Name Homo sapiens dihydrofolate reductase (DHFR), transcript variant 1, mRNA.
Background Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
Synonyms DHFRP1; DYR
Reference Data
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Folate biosynthesis, Metabolic pathways, One carbon pool by folate

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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