Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI7E3]

CAT#: CF500425

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Carrier-free (BSA/glycerol-free) SILV mouse monoclonal antibody, clone OTI7E3 (formerly 7E3)



  View other "OTI7E3" antibodies (4)

USD 545.00


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In Stock

Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI7E3
Applications FC, IHC
Recommended Dilution FLOW 1:100, IHC: 1:150
Reactivities Human
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human SILV (NP_008859) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 70.3 kDa
Gene Name Homo sapiens premelanosome protein (PMEL), transcript variant 3, mRNA.
Background Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Synonyms D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV
Reference Data
Protein Families Secreted Protein, Transmembrane

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