SNAIL (SNAI1) Mouse Monoclonal Antibody [Clone ID: OTI10D7]

CAT#: CF500366

Carrier-free (BSA/glycerol-free) SNAI1 mouse monoclonal antibody, clone OTI10D7 (formerly 10D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SNAI1 (Cat# RC204581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI1(Cat# TA500366). Positive lysates LY401811 (100ug) and LC401811 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-SNAI1 monoclonal antibody at 1:200 dilution.

Specifications

Product Data

• Clone Name: OTI10D7
• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SNAI1 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.1 kDa
• Gene Name: snail family transcriptional repressor 1
• Database Link:
  NP_005976
  Entrez Gene 20613 MouseEntrez Gene 116490 RatEntrez Gene 6615 Human
  UniProt ID O95863
• Background: The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2
• Synonyms: dJ710H13.1; SLUGH2; SNA; SNAH; SNAIL; SNAIL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction