BrdU Mouse Monoclonal Antibody [Clone ID: OTI3A4]

CAT#: CF190130

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Carrier-free (BSA/glycerol-free) BrdU mouse monoclonal antibody, clone OTI3A4 (formerly 3A4)



  View other "OTI3A4" antibodies (1)

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USD 495.00


Availability*
In Stock

Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI3A4
Applications IF, IHC
Recommended Dilution IHC 1:150, IF 1:150
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Note 5-chloro-2'-deoxyuridine (ClDU), 5-bromo-2'-deoxyuridine (BrdU), 5-iodo-2'-deoxyuridine (IdU) and 5-ethynyl-2'-deoxyuridine (EdU) are nucleoside analogs of thymidine. Cells that treated with these analogs will incorporate the chemicals into the genomic DNA during S-phase. Immunochemical method detection of these analogs is thus used to quantify the cell proliferation, cell cycle status in vitro or in vivo. Since the thymidine analogs can be passed onto the daughter cells, they can also used to trace dividing cell fate in a short period of time over 3 generations. In addition, combination of different analogs and their specific antibodies can be used to trace cell fate in different time frames.
Reference Data

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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