Cytokeratin 8 (KRT8) (+ KRT18) Guinea Pig Polyclonal Antibody

SKU BP5007

Cytokeratin 8 (KRT8) (+ KRT18) guinea pig polyclonal antibody, Serum

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: ELISA.; Immunoblotting: 1/2000 for Western blotting.; Cytological material.; Immunohistochemistry on Frozen Tissue: 1/100.; Immunohistochemistry on Paraffin-Embedded Tissue: 1/50 (microwave treatment recommended).; Incubation Time: 1 h at RT, extended with Paraffin.
• Reactivity: Bovine, Chicken, Hamster, Human, Mouse, Rat, Xenopus
• Antibody Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Keratin K8/K18 filaments, reconstituted from purified Bovine keratins K8 and K18
• Specificity: Reactive Polypeptide: Mr 52 500 and 45 500 Keratins K8 and K18 of simple type epithelium.; Tumors Specifically Detected: All adenocarcinoma tested, undifferentiated carcinoma, hepatocellular carcinoma, cervix carcinoma.
• Buffer: State: Serum; State: Liquid stabilized antiserum; Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: keratin 8
• Database Link:
  P05787
• Background: Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
• Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A

Reference Data

• Protein Families: Druggable Genome