Nucleophosmin (NPM1) Mouse Monoclonal Antibody [Clone ID: No-63]

CAT#: BM5524

Nucleophosmin (NPM1) mouse monoclonal antibody, clone No-63, Ascites

Specifications

Product Data

• Clone Name: No-63
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunoblotting: 1:1000 - 1:2000; ECL method; for blotting transfer nitrocellulose sheets are superior to nylon sheets.
  Immunoprecipitation: from Xenopus oocyte extracts nucleoplasmin - a 30 kD protein - is co-precipitated, which shows high sequence homology to NO38.
  Immunofluorescence microscopy.
  Immunohistochemistry on frozen sections: 1:300 - 1:500; 1 h at RT; also after fixation with paraformaldehyde.
• Reactivities: Chicken, Xenopus
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Nucleolar fraction prepared from Xenopus laevis oocytes
• Specificity: The antibody recognizes a 38 kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). No-63 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
• Formulation: State: Ascites
  State: Liquid Ascites containing 0.09% NaN3
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Database Link:
  UniProt ID P06748
• Synonyms: NPM, NPM1, Numatrin