Nucleophosmin (NPM1) Mouse Monoclonal Antibody [Clone ID: No-63]
Nucleophosmin (NPM1) mouse monoclonal antibody, clone No-63, Ascites
Product Datasheet for 'BM5524'
|Applications||IF, IHC, IP, WB|
|Recommend Dilution||Immunoblotting: 1:1000 - 1:2000; ECL method; for blotting transfer nitrocellulose sheets are superior to nylon sheets.
Immunoprecipitation: from Xenopus oocyte extracts nucleoplasmin - a 30 kD protein - is co-precipitated, which shows high sequence homology to NO38.
Immunohistochemistry on frozen sections: 1:300 - 1:500; 1 h at RT; also after fixation with paraformaldehyde.
|Immunogen||Nucleolar fraction prepared from Xenopus laevis oocytes|
|Specificity||The antibody recognizes a 38 kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). No-63 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.|
State: Liquid Ascites containing 0.09% NaN3
|Synonyms||NPM, NPM1, Numatrin|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.