p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: BP53-12]

CAT#: BM4079S

p53 (TP53) (16-25) mouse monoclonal antibody, clone BP53-12, Purified

Product Images

Confocal microscopy of human HeLa cells using anti-p53 (BP53-12; FITC). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.

Specifications

Product Data

• Clone Name: BP53-12
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western Blotting (Non-reducing conditions):
  Recommended Dilution: 1-2 µg/ml, overnight in 4°C.
  Positive Control: RAMOS Human lymphoma cell line
  Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
  SDS-PAGE: 12% separating gel.
  Immunoprecipitation.
  Immunocytochemistry.
  Immunohistochemistry on Frozen and Paraffin-Embedded Sections.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Bacterially expressed full-length wild-type p53.
• Specificity: This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.
• Formulation: Phosphate buffered saline (PBS), pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Precipitation Methods
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13