CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-25]
CD11a (ITGAL) mouse monoclonal antibody, clone MEM-25, Purified
|Applications||FC, FN, IP|
|Recommended Dilution||Flow Cytometry: 2 µg/ml.
Immunoprecipitation (excellent antibody for immunoaffinity purification of LFA-1 complex).
Functional Application (the antibody blocks binding of LFA-1 complex to ICAM-1).
|Immunogen||Leucocytes from a pacient suffering from a LGL-type leukaemia|
|Specificity||The antibody reacts with CD11a (a-subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.|
|Formulation||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State: Liquid Ig fraction
|Purification||Protein-A affinity chromatography|
|Storage||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.|
|Stability||Shelf life: one year from despatch.|
|Gene Name||integrin subunit alpha L|
|Background||CD11a (LFA-1a) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the aLb2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.|
|Synonyms||Integrin alpha-L, LFA1, LFA-1|
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